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gapdh polyclonal ab  (Proteintech)


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    Structured Review

    Proteintech gapdh polyclonal ab
    Gapdh Polyclonal Ab, supplied by Proteintech, used in various techniques. Bioz Stars score: 97/100, based on 6212 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    other:

    Article Title: Targeting periodontal inflammatory microenvironment to ameliorate periodontitis: A nitrogen ions implantation technique.
    Article Snippet: Periodontitis, an inflammatory disease affecting over 45 % of adults globally, causes irreversible alveolar bone loss through immune cells-mediated inflammation, thus necessitating novel regenerative therapies.. This study proposes a cold plasma-based nitrogen species implantation (PBNI) technique to regulate the periodontal immune microenvironment and ameliorate periodontal inflammation.. This study demonstrates that PBNI, optimizing for nitric oxide (NO) delivery, reprograms macrophage phenotype to resolve inflammation and reduce alveolar bone resorption.



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    Western blot analysis <t>of</t> <t>TFE3</t> protein expression in Vero cells following TOSV infection. ( A ) Cells were treated with 6.25, 12.5, and 25 μM of monensin for 4 h. ( B ) Cells were treated with 87.5 and 175 nM of brefeldin A for 12 h. ( C ) Representative blot images. Protein levels were normalized to <t>GAPDH</t> and expressed relative to mock-treated, uninfected controls. Statistical analysis was performed using one-way ANOVA, followed by Dunnett’s multiple comparisons test. Statistical significance is related to mock-treated, uninfected controls: **** p < 0.0001; ** p = 0.0052; ns = not significant.
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    Western blot analysis <t>of</t> <t>TFE3</t> protein expression in Vero cells following TOSV infection. ( A ) Cells were treated with 6.25, 12.5, and 25 μM of monensin for 4 h. ( B ) Cells were treated with 87.5 and 175 nM of brefeldin A for 12 h. ( C ) Representative blot images. Protein levels were normalized to <t>GAPDH</t> and expressed relative to mock-treated, uninfected controls. Statistical analysis was performed using one-way ANOVA, followed by Dunnett’s multiple comparisons test. Statistical significance is related to mock-treated, uninfected controls: **** p < 0.0001; ** p = 0.0052; ns = not significant.
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    Western blot analysis <t>of</t> <t>TFE3</t> protein expression in Vero cells following TOSV infection. ( A ) Cells were treated with 6.25, 12.5, and 25 μM of monensin for 4 h. ( B ) Cells were treated with 87.5 and 175 nM of brefeldin A for 12 h. ( C ) Representative blot images. Protein levels were normalized to <t>GAPDH</t> and expressed relative to mock-treated, uninfected controls. Statistical analysis was performed using one-way ANOVA, followed by Dunnett’s multiple comparisons test. Statistical significance is related to mock-treated, uninfected controls: **** p < 0.0001; ** p = 0.0052; ns = not significant.
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    Image Search Results


    Western blot analysis of TFE3 protein expression in Vero cells following TOSV infection. ( A ) Cells were treated with 6.25, 12.5, and 25 μM of monensin for 4 h. ( B ) Cells were treated with 87.5 and 175 nM of brefeldin A for 12 h. ( C ) Representative blot images. Protein levels were normalized to GAPDH and expressed relative to mock-treated, uninfected controls. Statistical analysis was performed using one-way ANOVA, followed by Dunnett’s multiple comparisons test. Statistical significance is related to mock-treated, uninfected controls: **** p < 0.0001; ** p = 0.0052; ns = not significant.

    Journal: Viruses

    Article Title: Antiviral Activity of Microbial Metabolites Monensin and Brefeldin A Against Toscana Virus: In Vitro Evaluation and Mechanistic Insights

    doi: 10.3390/v18030287

    Figure Lengend Snippet: Western blot analysis of TFE3 protein expression in Vero cells following TOSV infection. ( A ) Cells were treated with 6.25, 12.5, and 25 μM of monensin for 4 h. ( B ) Cells were treated with 87.5 and 175 nM of brefeldin A for 12 h. ( C ) Representative blot images. Protein levels were normalized to GAPDH and expressed relative to mock-treated, uninfected controls. Statistical analysis was performed using one-way ANOVA, followed by Dunnett’s multiple comparisons test. Statistical significance is related to mock-treated, uninfected controls: **** p < 0.0001; ** p = 0.0052; ns = not significant.

    Article Snippet: Membranes were blocked with 3% bovine serum albumin (Bio-Rad, Hercules, CA, USA) at room temperature for 1 h, followed by overnight incubation at 4 °C with primary antibodies against GAPDH (E-AB-40337, 1:1000, Elabscience, Houston, TX, USA) and TFE3 (14480-1-AP, 1:1000, Proteintech, Rosemont, IL, USA).

    Techniques: Western Blot, Expressing, Infection